Custom Monoclonal Antibody Services
High-affinity, high-specificity monoclonal antibodies — from antigen design to hybridoma development, delivered with fast turnaround and expert support.
Custom Monoclonal Antibody Services
High-affinity, high-specificity monoclonal antibodies — from antigen design to hybridoma development, delivered with fast turnaround and expert support.
- Mouse, Rabbit & Rat Monoclonal Antibody Development
- Full-Service: Antigen Design → Immunization → Screening
- ELISA, WB, IHC Validation Available
Common Challenges
- Low specificity or cross-reactivity
- Unstable hybridoma clones
- Long development timelines
- Poor reproducibility
Our Solution
QyaoBio specializes in custom monoclonal antibody production using optimized immunization strategies and high-throughput screening to ensure superior performance and reproducibility.
Monoclonal Antibodies ProductsPrinciple of Monoclonal Antibody Production
Monoclonal antibodies (mAbs) are homogenous antibodies produced by a single clone of B lymphocytes, directed against a specific epitope of an antigen. The core principle relies on Hybridoma Technology:
- B Cell Fusion: Antibody-producing B cells isolated from immunized animals are fused with immortal myeloma cells.
- Selective Breeding: The resulting hybrid cells (hybridomas) inherit both the specific antibody-secreting capability of B cells and the indefinite lifespan of myeloma cells.
- Clonal Expansion: Through rigorous screening, a single optimal clone is selected and cultured to harvest high-purity, highly consistent monoclonal antibodies.
Why Core Principles Matter?
By leveraging precise cellular fusion and modern high-throughput screening, we ensure that every batch of antibodies targets the precise conformational or linear epitope required, minimizing batch-to-batch variation.
Detailed Monoclonal Antibody Development Workflow
Our structured phase-by-phase approach ensures maximum transparency and success rates for your project.
Sequence analysis, epitope prediction, and peptide synthesis or recombinant protein expression with carrier protein conjugation (KLH/BSA).
Optimized immunization protocols across multiple host animals (Mouse, Rat, or Rabbit) to stimulate strong immune responses and high serum titers.
Harvesting splenocytes and executing PEG-mediated or electro-fusion with myeloma cells, followed by HAT medium selective culture.
High-throughput ELISA/Western Blot screening followed by sub-cloning via limiting dilution to secure stable, high-affinity monoclonals.
Scale-up via in vitro cell culture, followed by protein A/G purification and comprehensive validation (WB, IHC, or Flow Cytometry).
Why Choose QyaoBio?
Proven monoclonal antibody platform
Initial clones in 8–10 weeks
Research to large-scale production
ELISA / WB / IHC validation
Competitive vs US/EU CROs
Applications & Rationale
Monoclonal antibodies are indispensable tools across diverse scientific fields due to their unrivaled monospecificity.
Basic Diagnostics & Immunoassays
Applications: ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC).
Rationale: Their precise recognition of single epitopes eliminates background noise, yielding excellent signal-to-noise ratios and accurate quantification in complex biological samples.
Therapeutic & Clinical Drug Development
Applications: Targeted cancer therapies, autoimmune disease treatments, and neutralisation assays for infectious diseases.
Rationale: High specificity ensures targeted drug delivery to diseased cells while sparing healthy tissue, vastly reducing off-target toxicities.
Structure & Function Research
Applications: Immunoprecipitation (IP), Co-IP, enzyme inhibition studies, and receptor-ligand blocking assays.
Rationale: The ability to bind uniquely to active sites or specific conformations allows researchers to lock, map, and analyze structural interactions seamlessly.
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FAQ
Typically 8–12 weeks depending on project complexity.
Mouse, Rabbit, Rat supported.
ELISA, WB, IHC available.
From research to large-scale production.
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+86(021)-50795728
+86(027)-60707970